El siguiente estudio demuestra que el consumo de arveja transgénica en la presencia de un gran número de potenciales antígenos de la dieta en el tracto gastrointestinal induce una activa respuesta sistémica (Th2) inmunológica contra una arveja transgénica (RAI).

Abstract: The development of modern gene technologies allows for the expression of recombinant proteins in
non-native hosts. Diversity in translational and post-translational modification pathways between
species could potentially lead to discrete changes in the molecular architecture of the expressed
protein and subsequent cellular function and antigenicity. Here, we show that transgenic expression
of a plant protein (R-amylase inhibitor-1 from the common bean (Phaseolus vulgaris L. cv.
Tendergreen)) in a non-native host (transgenic pea (Pisum sativum L.)) led to the synthesis of a
structurally modified form of this inhibitor. Employing models of inflammation, we demonstrated in
mice that consumption of the modified RAI and not the native form predisposed to antigen-specific
CD4+ Th2-type inflammation. Furthermore, consumption of the modified RAI concurrently with other
heterogeneous proteins promoted immunological cross priming, which then elicited specific immuno-
reactivity of these proteins. Thus, transgenic expression of non-native proteins in plants may lead to
the synthesis of structural variants possessing altered immunogenicity.

Adjunto	Fecha
2-newproteins-immunogenecity.pdf	15/06/11 2:49 am